FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36137303
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT868620.24036658186694232No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT773550.21405858649717163No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC733480.20297032127715783No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA703010.19453859077419253No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC659700.18255374508717487No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA654100.18100409983556326No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG503970.13945977097405415No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC498530.13795440130106001No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG489120.1353504438336198No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT476890.13196612929304657No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC463420.12823867901818792No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT403610.11168791428624322No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG402890.11148867418246458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG123200.057.6867643
GCGTACG125150.056.8026582
GGCGTAC127950.055.986431
TCGGAAG180300.053.01177294
ACCGCGT152050.045.5503926
GTACGGA158300.044.9376684
ATCGGAA241800.043.55145694
AGACCGC163800.042.1936954
CCGCGTT165100.041.8930327
TACGGAA170500.041.6415
GCGTTCT197650.035.1602869
CAGACCG201950.034.5160523
GATCGGA357500.033.08447694
CGCGTTC215300.032.2778978
GACCGCG218600.031.769135
GCCGGCT223050.030.31626594
ACGGAAG236850.030.1147636
CGGAAGA246700.028.969517
AATACGG164100.026.77072132-33
TACGGAG169150.026.02549434-35
Produced by FastQC (version 0.11.5)