FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35521231
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1146160.3226689975918909No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC976620.27493979586461964No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC857960.24153442204747916No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG687630.19358281811798697No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG543120.15290010641804616No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG477940.13455051712594082No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC456270.12844994026248696No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC452350.127346374904631No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT429340.12086855886272636No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA394240.1109871445615159No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG389240.10957953568669959No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA384290.10818600290063145No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG371530.10459378505210024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG177350.061.20521594
GGCGTAC146700.057.5146141
GCGTACG146800.056.842822
CGTACGG148350.056.302783
GTACGGA186000.044.9565474
ATCGGAA269750.043.49754794
TACGGAA197500.042.291235
CGGAAGA258800.032.4813547
GATCGGA390800.032.12843394
ACGGAAG273500.030.7011766
ACCGCGT76600.028.7242016
AGATCGG567950.026.82319694
CCGCGTT84450.026.2211727
CGCGTTC87400.025.4887878
AGACCGC86300.025.4352514
GGTCGCC131450.022.44956690-91
CTCGCTA130850.020.9831166
GCGTTCT107750.020.5440439
TCGACCC223050.020.2301453
TCGCTAT136150.020.2008237
Produced by FastQC (version 0.11.5)