FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18546937
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1064570.5739869607579947No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT543660.2931265685541499No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT466590.2515725372874238No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA360320.1942746664853609No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG314820.16974231378475055No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA203250.10958682827250667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG25350.037.6237531
ACCGCGT9700.036.2924736
CCGCGTT10200.034.5133367
TGGAGTC322950.032.4133532
GGAGTCT341750.030.6967583
GACCGCG11450.030.3357265
TGCGCGG20200.028.8135097
GAGTCTT368750.028.6530274
GTGCGCG20900.027.848546
AGTCTTG405400.026.5605985
CGCGGAC22050.026.3959799
CGCGTTC13450.026.1736838
CTGGAGT477400.024.9875871
GTCTTGG430400.024.6142336
TCTTGGA487650.022.0806087
TCGCGCG12000.020.3398069
TTGGAAG519100.020.2183389
GCGTAAC22200.020.20035426-27
CTTGGAA525900.020.1622458
GCGCGGA31500.018.9242658
Produced by FastQC (version 0.11.5)