FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15540660
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT561480.3612973966356641No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA493880.3177986005742356No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG452820.2913775862801194No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC275380.17719968135201464No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG253100.16286309590454973No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC172280.11085758262519094No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC167890.10803273477445617No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA163830.10542023311751238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG67200.037.5656431
ACCGCGT11200.037.3576856
CTCGCTA36750.035.1802981
CCGCGTT12150.033.2759257
TCGCTAT43500.029.7208082
CGCGTTC14050.028.775988
TGGAGTC213150.027.4495752
GACCGCG15200.026.7147055
AGACCGC15400.026.6743624
GGAGTCT222800.026.2394053
GAGTCTT229000.025.5258774
TATACCC232800.024.31755694
AGTCTTG248250.024.1932035
GTCTTGG249750.023.849496
CAGACCG20100.020.8925673
TCTTGGA302650.019.898327
GCTATGT128550.019.8574962
CTTGGAA306800.019.0621998
TTGGAAG318200.018.4235929
TGCGCGG17600.017.8965827
Produced by FastQC (version 0.11.5)