FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15892729
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT348250.2191253622961796No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT256160.16118062542940234No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG205740.12945542581138833No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC165730.1042803913663915No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC162350.1021536326454695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC114300.049.994761
GGCGCGT39350.043.2315641
GCGTGCC134900.042.395112
GCGCGTG54350.031.3865932
CGTGCCT188000.030.4314693
GGCGATC15550.027.8031861
CCTGTAG291050.020.0119277
GCCTGTA296300.019.7524646
GCGATCT23900.018.4827692
TGTAGTC334100.017.385569
TGCCTGT343150.017.1651825
TGGCGCG27500.016.9176332
GTGCCTG341850.016.9144294
CGCGGGT9550.016.2385651
CGCGGTG14150.015.9412331
CGATCTG26850.015.9195443
GCTGCGA44850.014.1385924
CGACCCG6003.6379788E-1214.0914193
CGCGAAT3751.6530807E-512.5315811
TGTCCGC453350.012.52273494
Produced by FastQC (version 0.11.5)