FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23174157
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG587450.25349357907603715No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT504620.21775117860813661No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG348390.15033556560439287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC47350.046.837931
CGCGTGC47250.033.412821
GCGATCT66600.033.088362
CGATCTG81650.027.0468923
GCGTGCC64350.025.1911012
GGCGCGT21050.022.5447241
GGGCGAT35200.021.3576261
TCACCCC331450.018.9534853
GTCACCC326450.018.5673392
CGCGAGT7800.018.0718381
ACCCCAT359550.017.2097285
CGTGCCT93800.017.131643
CCCATTG353750.017.0403217
CCCCATT363950.016.7822116
TGTCACC365600.016.69471
CACCCCA388800.016.2896274
GCTGCGA132250.015.9158524
GGCTGCG146700.015.8842359
TGCGACA136400.015.3282726
CTGCGAC141250.014.8684825
Produced by FastQC (version 0.11.5)