FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14519133
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT565480.3894722914928873No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA354430.2441123722745704No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT225140.15506435542673244No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG213100.14677184925573722No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT197560.13606873082573182No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG184320.12694972902307597No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA162470.11190062106325495No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA158870.109421134168273No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC156480.10777503036854885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG29450.039.279711
TGGAGTC192500.031.1461182
GGAGTCT210850.028.636433
GAGTCTT221700.026.8105564
AGTCTTG245150.025.2627935
CGCGGAC13650.025.1093259
CTCGCTA18100.024.6810421
TGCGCGG13150.024.2784277
TCGCGCG8700.023.2056679
GTCTTGG267400.023.0708666
TCGCTAT20750.021.9825522
GTGCGCG15600.021.0670326
TATACCC277850.020.55058394
CTGGAGT402000.020.4588971
TCTTGGA315450.019.7058337
GGAGTGC218750.019.1323975
CTTGGAA316000.018.9439038
ACCGAAG126000.018.8884394
TTGGAAG324300.018.4010879
GCGTAAC14550.017.4686126-27
Produced by FastQC (version 0.11.5)