FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16698547
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1207800.7232964640576214No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT764050.45755478006559497No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA506670.303421609077724No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT465200.2785871129985142No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA420130.25159674072241134No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG299450.17932697976656292No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG276730.16572100554617117No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC273280.16365495752414866No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA204400.12240585962359479No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC204050.12219626054889687No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG198860.11908820569837603No Hit
AGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTG181600.1087519770432721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG10950.044.6692965
CGCTATG35950.041.994981
ACCGCGT11650.041.9113856
CCGCGTT12450.039.2182857
TGGAGTC344800.037.3469242
GGAGTCT385400.033.4859283
CGCGTTC15550.031.3995678
CGCGGAC39450.031.0610989
TGCGCGG39450.030.8231727
GAGTCTT422200.030.6450064
AGTCTTG448800.029.1851715
GTCTTGG460050.028.4417656
GTGCGCG42500.028.390226
CTGGAGT596800.026.739071
AGACCGC19550.025.5003684
TCTTGGA522050.025.306767
GCGTAAC42300.023.31466326-27
CTTGGAA558900.023.0751888
CGGACTT57600.022.86554394
TTGGAAG566600.022.8197389
Produced by FastQC (version 0.11.5)