FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17409863
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG249540.14333254661452532No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC234740.13483161814656439No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT191040.10973090368373375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT32950.045.2324141
CGCGTGC82950.039.1091731
GCGCGTG47250.032.040552
GGCGATC20700.031.571151
GCGTGCC105250.030.867532
GCGATCT27850.023.8034442
CGTGCCT145450.022.533
ACTCCGC23050.021.009155
CGATCTG40500.016.9488413
TGGCGCG21350.015.855522
CGTAGGG49100.015.73007280-81
GCCTGTA226950.014.7498286
CGCGGGT8350.014.6397211
CCCATTG182650.014.594887
CCTGTAG227150.014.4884687
TCACCCC198350.014.3168323
ACGTAGG56000.014.12829180-81
TCGCGCG3709.3496965E-713.9774557
AACGTAG57550.013.87048178-79
CCCCATT190950.013.8373796
Produced by FastQC (version 0.11.5)