FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21580178
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG249780.11574510645834339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC73800.035.8694341
GGCGCGT32150.031.150941
GCGTGCC101650.026.3194242
GGCGATC23250.025.8856771
GCGCGTG47900.021.300792
CGACCCG15350.019.6038823
CGTGCCT147500.018.3293193
GCGATCT37750.015.9427962
GCGACAT69200.015.0046387
CGATCTG43050.014.7445353
TCACCCC211800.013.9191293
GCTGCGA74850.013.756974
CGTGCGG21250.013.7079038
CCGTGCG21000.013.6473987
TGGCGCG30700.013.6308562
CGTAGGG46400.013.572535580-81
GTCGACC24050.013.4898311
GGCTGCG78500.013.4167973
TGCGACA78550.013.1690676
TGCGCGA7150.013.1420249
Produced by FastQC (version 0.11.5)