FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15517819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG456960.29447437168844415No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT346560.22333035331833678No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT278340.1793679897928955No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC263530.1698241228358186No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA204920.13205463989494917No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT202200.13030181625394652No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG201360.1297605030706957No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG194380.12526244828606392No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA177160.11416552802942218No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA170920.11014434438241612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC31650.050.9162061
GGCGCGT51350.044.374851
CGCGTGC125400.043.3857041
GCGTGCC146050.037.283452
GCGATCT43750.036.4045682
GCGCGTG69550.032.6950842
CGATCTG54650.029.0556093
CGTGCCT196000.027.8757843
ACTCCGC20350.026.548315
CCTGTAG280300.019.7602657
TCACCCC240300.018.9440783
GCCTGTA300950.018.93526
GTGCCTG303500.018.3272154
CGCGGTG15050.018.1061941
TGGCGCG34000.017.9625453
CGCGAGT6100.017.7146951
GGGCGAT27850.017.7133121
CCCATTG252700.017.6054367
GTCACCC254900.017.5285072
TGTAGTC316700.017.4161229
Produced by FastQC (version 0.11.5)