FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10470356
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT752340.7185429034122622No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT251500.24020195683890785No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA229760.21943857496344918No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT220500.21059455858043413No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA204730.19553298856313958No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG153060.1461841412078061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTC189500.038.4565542
GGAGTCT209200.034.8537983
GACCGCG3000.032.8866925
CGCTATG16300.032.5730631
GAGTCTT232200.031.1991124
AGTCTTG242250.030.467345
GTCTTGG243100.030.1289046
TGCGCGG8000.029.9503827
CGCGGAC8100.029.000759
CTGGAGT305150.027.0228731
GTGCGCG8900.026.921696
TCTTGGA287550.025.8636427
ACCGCGT4000.025.8395446
TATACCC288450.024.44479694
TTGGAAG301850.024.1871159
CTTGGAA303100.024.1803678
CTCGCTA9550.022.6319641
TCGCGCG4300.021.8517279
CCGCGTT4600.021.4478447
CGCGTTC4750.020.7706438
Produced by FastQC (version 0.11.5)