FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17387653
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG245580.14123815330338144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC26750.039.3715171
CGCGTGC49600.034.4098551
GGCGCGT21050.032.1638681
GCGTGCC64200.026.5844842
GCGATCT40200.026.1986312
CGATCTG45900.023.1500763
GCGCGTG32800.021.0717282
CGTGCCT92350.018.9392453
TGCGACA84350.015.7745416
GCTGCGA85500.015.7274334
GCGACAT86250.015.4815577
CTGCGAC95250.014.2162615
TCACCCC213100.013.5248873
CGTAGGG35250.013.15863380-81
CTTAGCG14300.013.1516991
CGACATC105000.012.8836788
CCCATTG208600.012.6220277
GTCACCC220850.012.2412872
GGCTGCG109900.012.2355933
ACGCACG36700.012.15896894
Produced by FastQC (version 0.11.5)