FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15656830
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG284090.1814479687139734No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC191040.1220170366542908No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT169150.10803591787098665No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA166180.10613898215666902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC23200.033.8306161
GGCGCGT49100.033.6932261
CGCGTGC96350.030.193981
CGACCCG26800.028.9353833
ACTCCGC24500.025.5135655
GCGCGTG67300.024.9305482
CGTGCGG33050.024.0261578
GCGTGCC125150.023.621012
GCGATCT33850.023.1865672
CCGTGCG34550.022.9830537
GTCGACC36050.022.8146721
TCGACCC37250.022.0795612
GACCCGT34950.021.7844454
ACGCGCG27350.019.58437332-33
GGTCGAC19650.018.6559645
CGATCTG45400.017.9089583
CGTGCCT165050.017.7399583
CGACCTA14050.017.7287544
GTGCGGA45600.017.3106569
TGGCGCG39000.017.2326283
Produced by FastQC (version 0.11.5)