FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16029091
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT18800.035.5141531
CGCGTGC40800.025.814211
GCGCGTG27250.024.6740532
CGCGGGT10250.022.0185321
GCGTGCC55800.019.296222
TCGTCGG14650.018.11147114-15
GGCGATC17900.014.9724471
CGTACGC1950.004777989414.4669437
ATCGTCG17050.014.04716514-15
CGTCGGG17450.013.86301416-17
CGCGAGT6159.276846E-1112.997051
ACGCGCG6050.012.80844932-33
CGTGCCT87300.012.4951263
GACTCGT23250.011.95568826-27
AGCGCGA19950.011.77245194
GCGATCT22800.011.7546852
ACGCACG19200.011.7430294
GCGGGTC20450.011.72594452
TCGTAGC74800.011.36623994
CGACCCG9400.011.0042823
Produced by FastQC (version 0.11.5)