FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14333138
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT610410.42587324562144036No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA563770.39333326728592166No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG334000.23302643147648477No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG306830.21407035919140666No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA248470.17335352523641367No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT229000.1597696191859731No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC222420.15517885894910102No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT188000.13116457819634472No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA180570.1259807866218828No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC177720.12399238743114034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG44450.040.5228231
TGGAGTC226650.030.2741682
GGAGTCT229200.029.7734053
CTCGCTA23250.027.7121521
GAGTCTT256900.026.3986324
AGTCTTG275250.025.3906155
GTCTTGG287550.023.9526716
CGCGGAC24300.023.7654849
TGCGCGG25050.023.2410477
TCGCTAT28050.022.9699672
GTGCGCG26550.021.9277656
TATACCC302550.021.14252994
CTGGAGT525200.020.3538881
CTTGGAA341800.020.0276628
TCTTGGA355200.019.8667937
TTGGAAG348000.019.3471819
GCTATGT94250.019.260992
GCGTAAC24450.018.96433826-27
GGAGTGC324500.018.4789495
GAGTGCA342250.017.5865966
Produced by FastQC (version 0.11.5)