FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14415600
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT564770.39177696384472377No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA323900.22468714448236632No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT215570.14953938788534643No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT195260.13545048419767475No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG175050.12143094980437859No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC167930.11649185604484032No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA166810.11571491994783428No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA166010.11515996559282998No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG159740.11081051083548378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG23350.036.01621
TGGAGTC187350.030.1427252
GGAGTCT200650.028.1965753
GAGTCTT216450.025.9215094
AGTCTTG235550.024.6567045
GTCTTGG250150.022.8985396
TATACCC252050.021.59169694
CTGGAGT362650.021.3631211
TGCGCGG11050.020.3951747
ACCGAAG113950.019.09548694
CTTGGAA301250.019.0611638
TCTTGGA307450.019.043227
CGCGGAC11800.018.7011079
GGAGTGC186950.018.559555
TTGGAAG308400.018.4213929
GTGCGCG12350.018.2483126
CGCACTA65750.017.66098478-79
CTCGCTA15000.017.5399551
GACCGCA49350.017.26783618-19
CGATCGG68950.016.68547466-67
Produced by FastQC (version 0.11.5)