FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14415600
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG251660.17457476622547796No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT176190.12222175976025973No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT154160.10693970420932877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC63600.042.339711
GGCGCGT26250.038.4910281
GGCGATC19900.034.478641
GCGTGCC82500.032.9243132
GCGCGTG35400.028.6743342
GCGATCT24300.027.8483242
CGTGCCT111550.024.4653823
GCTGCGA68100.019.1063674
CGATCTG36500.019.0464613
CGCGGGT5850.018.4766121
GGCTGCG71800.017.9909343
TGGCGCG18600.016.6677283
CCTGTAG168150.016.5933887
CTGCGAC79650.016.394745
GCCTGTA172400.016.1843266
GCGACAT80850.016.0933047
CGCGGTG10950.015.8795411
TGTAGTC175550.015.6069529
TGCGACA86500.015.2593366
CGTAGGG28450.015.18978980-81
Produced by FastQC (version 0.11.5)