FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14819219
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT449770.30350452341651746No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA318490.2149168589788706No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT271920.18349145120265783No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA196370.13251035698979818No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA178190.1202425040078023No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT165290.11153759182585804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG22650.035.6820451
CCGCGTT7800.028.3106787
GACCGCG7950.027.7765125
ACCGCGT8400.026.2884856
TGGAGTC174300.025.9877782
GGAGTCT180500.025.0405243
TCGCGCG10850.023.816699
CGCGTTC9600.023.0024248
CTCGCTA14400.022.8415221
TGCGCGG14950.021.9990467
CGCGGAC14600.021.8828839
GAGTCTT218450.020.9914934
AGTCTTG224150.020.8140955
ACCGAAG117150.020.60684894
GTCTTGG223300.020.367316
GTGCGCG16700.019.9750966
TCGCTAT16400.019.7694572
CGCACTA71200.018.58081878-79
CCTCGCG15850.017.785627
CTGGAGT339250.017.659561
Produced by FastQC (version 0.11.5)