FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20539349
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT673280.32780006805473727No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA469990.22882419496353074No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT294130.14320317552420966No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG289390.14089541007361042No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG237710.11573395047720354No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA225450.1097649200079321No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT224400.10925370614229303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG55950.034.5821041
ACCGCGT14350.032.0720256
GACCGCG15300.030.387575
CCGCGTT16050.028.6749867
TGGAGTC252650.026.209222
CGCGTTC17700.025.7366318
GGAGTCT263500.025.4507663
GAGTCTT280850.023.8617784
AGACCGC20400.023.481364
CTCGCTA36650.022.2961351
AGTCTTG308400.022.1260265
GTCTTGG306750.021.9847776
TCGCTAT39200.020.3665352
TCTTGGA383700.018.3223937
TATACCC341350.018.20888794
CTTGGAA376300.017.9963448
CTGGAGT556400.017.6828081
TTGGAAG386900.017.3093369
CAGACCG30750.016.4942233
TGCGCGG20550.015.9969947
Produced by FastQC (version 0.11.5)