FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14921054
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG411190.2755770470370257No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT334380.22409945034714035No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC283450.18996647287785434No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT269200.18041620920345172No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA226800.151999986059966No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG197080.13208182210184347No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG195420.13096930015801833No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT183070.12269240497353605No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA175810.11782679695415618No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA162600.10897353497949942No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG157680.10567618078454781No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC152900.10247265374148501No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC151540.10156118998027887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC117450.044.5961881
GGCGATC31200.044.456271
GGCGCGT47050.039.872991
GCGTGCC140100.037.621112
GCGATCT41350.032.9751362
GCGCGTG63200.030.0558092
CGATCTG49350.027.8199843
CGTGCCT188750.027.7995933
ACTCCGC17750.022.7803975
GGGCGAT24500.022.2616581
CCTGTAG275050.019.623657
CGACCCG8900.019.5466773
GCCTGTA286450.019.2366646
GTGCCTG286100.018.6361584
TGGCGCG34250.018.2579613
GCTGCGA90700.017.625154
TCACCCC225650.017.523543
GGCTGCG93250.017.2944373
TGTAGTC312550.017.1407669
TGCCTGT328750.016.733295
Produced by FastQC (version 0.11.5)