FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30956901
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT906010.2926681840666157No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC752540.24309280828852992No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC446230.14414556547504545No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC426430.1377495764191642No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT412340.13319808723747897No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG364490.11774111368576591No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA359750.11620995266935796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG83450.053.2134552
CGTACGG83400.053.201353
TCGGAAG161300.052.6739594
GGCGTAC97250.046.6301161
GTACGGA105700.042.0216944
ATCGGAA215750.041.34064594
TACGGAA110200.040.255295
AGACCGC76500.032.6863864
GATCGGA302800.032.09419694
ACCGCGT81050.030.8453546
CCGCGTT84050.029.576667
CGCGTTC96150.026.0526498
ACGGAAG172650.025.993696
AGATCGG428950.024.74807494
CGGAAGA182200.024.6312317
CAGACCG107050.024.0169243
GCGTTCT106950.023.422949
TATACCC350500.023.39587294
GACCGCG109500.022.7025225
GGTCGCC138950.022.1697190-91
Produced by FastQC (version 0.11.5)