FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30956901
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC833410.26921622419505103No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC547000.17669727341247757No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT480940.1553579281078555No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC424330.1370712139435404No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG370170.11957592266745305No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT336750.10878026841252618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT105650.046.5856131
TCGGAAG183600.046.08458394
GGCGATC109050.044.7617872
ATCGGAA233800.037.77704294
GCGATCT158600.030.989713
GATCGGA326900.029.59075594
CGATCTG179350.027.3770774
AGGTCGA238100.024.3322436
GGTCGAC362250.023.7599351
AGATCGG461150.023.0036394
TCGACCC389300.022.1845253
CGGTGGC296350.021.4185491
CTTAGCG46950.021.1175548
CGACCCG411100.021.0415884
GTCGACC412450.020.8306562
ACCCGTG417850.020.567046
GGCGCGT250850.020.0045744
TTAGCGG50400.019.8583099
CCGTGCG452950.019.2126568
TGGCGCG269200.018.6765963
Produced by FastQC (version 0.11.5)