FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7624575
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG153430.2012308882790188No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT130960.17176039320224407No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT109550.14368013954876174No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG83230.1091601826987078No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC80640.10576327205122908No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA76350.10013672893243229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT22800.040.6072651
CGCGTGC49700.039.3377341
GGCGATC12700.038.485931
GCGTGCC62500.031.3563562
GCGATCT15600.031.331292
GCGCGTG30500.030.8176632
CGTGCCT78950.024.508593
CGCGAGT2602.910383E-1123.4986231
CGATCTG24350.020.0589223
TGGCGCG15200.018.229793
CCTGTAG111600.017.6329887
TCACCCC96700.017.5329073
CTTAGCG6650.016.961411
TAGCGGC5550.016.9242823
GTCACCC98600.016.7778112
GCCTGTA118650.016.6248466
ACCCCAT105150.016.3027065
CGTAGGG23650.016.2838680-81
TGTAGTC119700.016.176169
CCCCATT106050.015.7657796
Produced by FastQC (version 0.11.5)