FastQCFastQC Report
Wed 8 May 2024
EGAF00004723841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221793
Total Bases522.1 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTTTTGAGCAGCTTGGAGAGTACAAATTCCAGAATGCGCTATTAGTT90710.17371427783521867No Hit
CCAGAATGCGCTATTAGTTCGTTACACCAAGAAAGTACCCCAAGTGTCAA89490.17137791559336035No Hit
GCTTTTTGAGCAGCTTGGAGAGTACAAATTCCAGAATGCGCTATTAGTTC83180.15929394367030636No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG82880.15871942836493136No Hit
CAAAATTGTGAGCTTTTTGAGCAGCTTGGAGAGTACAAATTCCAGAATGC79520.15228485694473146No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG78310.1499676452130523No Hit
CTCATGAATGCTATGCCAAAGTGTTCGATGAATTTAAACCTCTTGTGGAA71900.13769216818820662No Hit
ATTTAATCAAACAAAATTGTGAGCTTTTTGAGCAGCTTGGAGAGTACAAA59390.11373487995406942No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA56230.10768331873745284No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT54370.10412132384412787No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA52450.10044442588972792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)