FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15088280
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC37550.039.549161
GGCGCGT16350.033.0552331
GCGTGCC53450.028.1359082
GGCGATC18150.024.0805531
GCGCGTG23950.023.3507122
CGTGCCT78200.019.4596463
GCGATCT23900.018.0904182
CGATCTG25150.016.9941753
ACTCCGC16350.015.5121765
ACGCGCG4750.013.34867932-33
AAGCGCG7500.013.1590318
TTCGATC10700.012.737172
CGACCCG6101.1532393E-912.3193243
GATCGTA79700.011.67627994
TCACCCC122800.011.6271133
GCGACAT37700.011.5861657
AGCGCGT8551.8189894E-1211.5430119
CGGATCG94850.011.39693594
CGTAGCC56750.011.34624994
GCTGCGA40600.011.3369644
Produced by FastQC (version 0.11.5)