FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758142
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT420940.2511853641054002No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT257110.15342392969339919No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA212360.12672049204500116No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT180630.10778641212134377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG22450.035.3699381
GACCGCG11900.025.6611395
CCGCGTT12000.025.4472967
ACCGCGT12250.025.3115446
CGCGGAC28050.025.1229429
TGCGCGG28700.025.0448867
GTGCGCG29850.024.0800786
CGCGTTC13250.023.4013088
TGGAGTC193100.022.4343052
GGAGTCT192500.022.3549793
GAGTCTT203650.021.3155754
GCGTAAC27450.020.11027526-27
AGTCTTG232800.019.5546635
GTCTTGG234600.018.6637426
GGTGCGC40800.018.0779425
CGGACTT41650.017.77689694
GCGCGGA42200.017.2556518
CGGGATA32150.017.17712476-77
ACCGAAG149550.016.71324394
GCGGACT33500.016.68184992-93
Produced by FastQC (version 0.11.5)