FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15649197
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT857140.5477213942670669No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT459100.29336968535829666No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT390690.24965498229717475No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA284640.18188792690129724No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG258510.16519058453925783No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA194560.1243258679662605No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC161130.10296374951379295No Hit
CTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGAC156540.10003069167063333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG23800.037.1505851
TGGAGTC274050.033.945752
GGAGTCT296200.031.502443
GAGTCTT314950.029.4478954
AGTCTTG346800.027.4352265
GTCTTGG367800.025.7703596
TGCGCGG22700.025.6444327
CTGGAGT418850.025.107191
GACCGCG8850.024.9773485
CGCGGAC22700.024.610079
GTGCGCG24150.023.7158436
ACCGCGT9150.023.6011226
CCGCGTT9600.022.983917
TCTTGGA415500.022.9023487
TTGGAAG446250.020.8505339
CTTGGAA447200.020.753958
TCGCGCG14150.020.5696549
CGCGTTC11700.019.2597778
TATACCC502550.017.41023694
ACCGAAG262300.017.395894
Produced by FastQC (version 0.11.5)