FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15649197
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG450500.2878741957175183No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG305690.19533909631273733No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA292860.18714059258120402No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT242480.15494724745301627No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA237160.15154771200081385No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA219750.14042254053035436No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG206300.13182785033634634No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG168570.10771798706348959No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC158710.10141734428929484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC29050.042.728811
GGCGCGT16350.033.070681
CGCGTGC36300.032.8995631
GCGATCT39600.031.9390142
GCGCGTG22350.025.0342062
CGATCTG53200.023.7740763
GCGTGCC55850.021.635872
GCTGCGA114300.018.7577884
CGTAGGG73950.018.72208680-81
GGCTGCG118150.018.5445023
TGCGACA124400.017.3099966
AACGTAG80950.016.92847378-79
TCACCCC276600.016.8965193
GCGACAT130650.016.6258187
ACGTAGG82800.016.60724680-81
GTCACCC272400.016.5874922
GAACGTA82250.016.546478-79
CTGCGAC131550.016.3696355
CGTGCCT75100.016.2778283
ACCCCAT287650.015.9205845
Produced by FastQC (version 0.11.5)