FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14838545
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG333990.22508271532013413No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT200600.13518845681972189No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT160670.1082788103550584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC53100.045.320461
GGCGATC23950.036.502811
GCGTGCC69550.034.939032
GCGATCT31550.028.00762
CGTGCCT98850.024.9631883
CGCGAGT7800.021.6933571
GCGCGTG26850.021.3567141
CGATCTG43100.021.0473523
TCACCCC184100.019.7352713
GTCACCC184150.019.372582
CCCATTG189250.018.9744437
ACCCCAT201450.018.6187955
CCCCATT202800.017.8920826
GGCGCGT15500.017.891191
TGTCACC209000.017.4740521
CACCCCA232900.016.0238534
CCTGTAG157350.015.9210617
CTACGCA16750.015.7060928
CGAAGCT205600.015.58334294
GCCTGTA167300.015.3955856
Produced by FastQC (version 0.11.5)