FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18057169
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG785870.4352121863621036No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA712570.3946188907020807No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT586840.32499003581347663No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC568340.3147447974818201No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT519120.28748692555294797No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG371100.2055139429663642No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG350810.1942774086015366No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA333480.18468011236977402No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC330800.18319593730335026No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA318630.17645623187112No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG298870.16551320973957767No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG276970.15338506274156263No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT271210.15019519394208472No Hit
CTTGACTCCGCTGCTCGCCATGTCTTCTCACAAGACTTTCAGGATTAAGC239240.13249031451164908No Hit
CTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCC235680.13051879837863842No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA232850.12895155381222825No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC230520.12766120757910612No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG229820.1272735499125029No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC214510.1187949229472239No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC212370.11760979808075119No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA207970.11517309274781666No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC198190.10975696134870312No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG192190.10643418134924695No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT189780.10509953138279872No Hit
CCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCG188440.10435744384958684No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTC184930.10241361754990497No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCA184140.1019761181833099No Hit
GCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGT182290.10095159435014427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC34800.052.0040171
CGCGTGC165800.048.6505931
GGCGCGT54800.045.8911131
ACTCCGC56500.042.2640085
GCGTGCC194600.041.450512
GCGATCT44600.040.3663332
GACTCCG64300.037.429534
GCGCGTG79850.031.8476772
CCGCTGC81050.031.1796078
TCCGCTG82950.030.6002067
CGTGCCT265650.030.435053
CTCCGCT83450.029.9662426
AGCTTCC225750.029.73431294
CGATCTG63500.028.4258123
CGCGAGT6600.025.6397631
CGCTGCT99400.025.4686159
CTTGACT129000.024.0132581
TTGACTC112000.023.9647832
TAAGCGA63000.023.37254146-47
TGACTCC117250.023.0120053
Produced by FastQC (version 0.11.5)