FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16761699
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC182260.10873599388701587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT26850.043.756621
CGCGTGC68600.040.1440851
GCGTGCC87700.031.6691152
GCGCGTG40400.029.4297942
GGCGATC17000.026.2616941
CGTGCCT128800.021.6730443
ACTCCGC13900.016.2284035
CGATCTG28350.016.0793323
GCGATCT28100.015.7206182
GTCTACG56350.013.7606221
CAGCGCA35800.012.8605339
TCTACGC61500.012.8375522
CGCGGGT8450.012.7914321
CCTGTAG221250.012.7441497
TACGCCT63300.012.4725414
GCCTGTA237650.012.4183736
CGACCCG7207.2759576E-1212.3978048
CTACGCC67850.011.982453
CGTAGGG22500.011.81546580-81
CTGCGAA68650.011.6338729
Produced by FastQC (version 0.11.5)