FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24410608
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG297490.12186914803596863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC34600.036.013111
CGCGTGC109000.034.855851
GGCGCGT46950.030.8465061
GCGTGCC141400.027.0352952
GCGATCT52700.023.7334562
CGACCCG28400.021.6891253
CGATCTG59100.020.8450473
CGTGCCT194600.019.7409613
GCGCGTG73550.019.6905572
GTCGACC39150.017.535251
CGTGCGG40650.017.1060038
TCGACCC41000.016.514622
CCGTGCG40650.015.9502567
ACGCGCG26800.015.42788332-33
GACCCGT42850.015.2529214
TGCGACA100000.014.3887186
CGTAGGG50750.014.12230880-81
GGTCGAC22700.014.0858425
GCTGCGA109050.013.7979124
GCGACAT105600.013.79262357
Produced by FastQC (version 0.11.5)