FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19437758
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG862880.4439195096471517No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA766580.39437675888340623No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT635330.32685353938453193No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC604740.31111612769332764No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT558340.28724506190477317No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG397730.20461721974314118No Hit
CCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAAGCTGCGCGCTCGGTCG377110.19400900042072755No Hit
GTTGATTCGGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCA364330.18743416807638No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC355120.18269596730240184No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA342750.17633206463420317No Hit
CCCGAAGCTGCGCGCTCGGTCGAAGAGGACGACCATCCCCGATAGAGGAG322670.16600165512915638No Hit
CTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTG299200.15392721732619574No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT290250.14932277683465345No Hit
CTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCC254310.13083299010101884No Hit
CTTGACTCCGCTGCTCGCCATGTCTTCTCACAAGACTTTCAGGATTAAGC253680.13050887864742425No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA253200.13026193658754265No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC249420.12831726786597508No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG246500.12681503700169536No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC230520.11859392425813718No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC224250.11536824360093381No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA220900.11364479380801017No Hit
GTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGGC214960.1105888858169754No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG208180.10710082922114783No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT202120.10398318571514266No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTC201190.10350473547412206No Hit
CCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCG200600.10320120252551761No Hit
GGCTGATCTGGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCA200360.10307773149557681No Hit
GCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGT195640.10064946790674109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC37450.050.341051
CGCGTGC177400.049.6114541
GGCGCGT62350.045.1668781
ACTCCGC60200.043.0312275
GCGTGCC208100.042.337482
GCGATCT47250.039.5017362
GACTCCG69000.037.3387764
GCGCGTG86250.033.0324632
CGTGCCT286450.030.9050163
TCCGCTG88450.030.6157867
CCGCTGC86800.030.5765428
CTCCGCT90750.029.4254066
AGCTTCC241850.028.64896494
CGATCTG70950.026.7042313
CGACCTA11750.026.0078284
CGCTGCT105750.024.5552649
TAAGCGA66300.024.11754246-47
CTTGACT134500.023.8392071
TTGACTC118850.023.4575672
GCTGCGA155050.023.0446664
Produced by FastQC (version 0.11.5)