FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21138929
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT434780.20567740210490323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT9000.026.1059327
ACCGCGT9100.025.8190576
CGCTATG25500.025.799111
GACCGCG9350.025.1287085
TCGCGCG6200.024.2532569
CGCGTTC10700.021.9582638
TGGAGTC206500.021.0265392
GGAGTCT213300.020.4442863
GAGTCTT233500.018.5347864
AGTCTTG250000.018.1196065
GTCTTGG245700.017.8629636
TATACCC230350.017.72934394
TGCGCGG8200.016.0455997
ACCGAAG97750.015.81758794
TCTTGGA297850.015.2244447
CGCGGAC8350.015.1945929
CACCCGA88250.014.96417194
CGATCGG53850.014.74835566-67
CGCACTA54950.014.58174678-79
CTGGAGT374850.014.5167021
Produced by FastQC (version 0.11.5)