FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21138929
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGC38950.036.066861
GGCGCGT17150.034.5184331
GGCGATC25850.027.8083671
GCGTGCC57150.024.9919362
GCGCGTG25000.024.243332
GCGATCT37850.018.3712352
CGATCTG38750.018.308163
CGTGCCT84700.016.807333
CGCGCGT4502.6811904E-914.6149898
GGGCGAT25150.011.9560181
TCACCCC172550.011.8716483
GGCTGCG66450.011.4525729
CGAAGCT154100.011.21806394
CGGATCG74400.011.04937794
GCGACAT55100.010.9129817
ATGCGCG3450.001452013310.8932116
GTCACCC182250.010.5953882
TGCGACA56950.010.5584776
CTTGGAT174200.010.4107911
CCCATTG186650.010.3442467
Produced by FastQC (version 0.11.5)