FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8703739
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT590170.6780649098048551No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT210170.24147093565190775No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA187200.21507997884587302No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT181780.20885277005663888No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA175940.20214301003281462No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG119180.13692965747249544No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG102450.11770803329465647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTC169150.038.4274832
GGAGTCT183250.035.445253
CGCTATG15950.033.026361
GAGTCTT199600.032.7067454
AGTCTTG216850.030.4738835
GTCTTGG220500.029.9153756
TCGCGCG5450.029.2896469
CTGGAGT268250.027.8428781
ACCGCGT3350.026.6278156
CGCGGAC8150.026.4991519
TCTTGGA252600.026.2254497
CCGCGTT3050.026.1684687
TGCGCGG8650.026.0527957
GACCGCG3400.024.9002955
CTTGGAA261900.024.8998078
TTGGAAG263800.024.5960279
CCTCGCG7000.024.1453597
TATACCC267100.022.94504594
GTGCGCG10250.022.901976
CTCGCTA10350.022.2668651
Produced by FastQC (version 0.11.5)