FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16882022
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC350080.20736852493143296No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT294140.17423268373895023No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG200800.11894309816679542No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT170770.10115494459135285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGT55200.038.2251471
CGCGTGC124750.037.1054421
GCGTGCC151850.030.6689932
GCGCGTG80750.026.4794832
CGACCCG20600.025.3219053
CGTGCCT209400.022.3523563
CCGTGCG24750.021.8318847
GGTCGAC15700.021.5516195
GTCGACC25050.021.3864671
CGTGCGG25150.021.1110128
TCGACCC28100.019.3995782
TGGCGCG40300.017.2582723
GGCGATC17150.016.7150481
GACCCGT32250.016.174714
GTGCGGA35000.015.8409999
ACGCGCG22200.015.66198232-33
CCTGTAG314650.015.0970487
GCGACAT32400.014.6468747
AGGTCGA26050.014.6123654
ACCCGTG37950.014.2407585
Produced by FastQC (version 0.11.5)