FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19448184
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT477040.24528768341558266No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT307180.15794790917239368No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA266540.13705135656881898No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT212880.10946009149234706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG31350.035.374591
CGCGGAC37450.026.6007799
TGCGCGG38250.025.9216377
GTGCGCG39600.025.3940016
TGGAGTC218200.022.504862
GCGTAAC36400.021.6882726-27
GGAGTCT227100.021.4986383
GACCGCG13750.020.5050625
GAGTCTT236400.020.4938584
CGGACTT49850.019.70342394
ACCGCGT14400.019.2531626
CCGCGTT14500.019.1203337
AGTCTTG272350.018.9446895
GGTGCGC54200.018.9003455
GCGGACT41800.018.49472892-93
CGGGATA42650.018.40056676-77
CGCGTTC14650.018.2828168
GTCTTGG274250.017.9224596
GCGCGGA57250.017.8110738
ACCGAAG181300.017.34158994
Produced by FastQC (version 0.11.5)