FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26948953
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT30550.017.96851334
CATCGTT31550.017.61291132
CGTGCGA20800.016.98091110
TCCGAGC78600.016.60515445
GCGAAGT26900.016.30856932
CGGTATG26100.015.77829743
CGAGAAT24100.013.90893614
CGGTCGT22400.013.35759510
TATACCG28550.012.9229726
TCGTTCG16200.012.63788523
TTCGGTA16500.012.54445826
TCCGTAT25900.012.33426413
TATACTG32000.012.2327465
GTACAAG36100.012.1989951
CCGTGCG38300.012.1589559
TATACAC452850.012.04027845
CCACGAC56600.012.0037019
GCGCTAT26600.011.92536819
TCTCCGA108250.011.82861643
AACCACG52600.011.7617427
Produced by FastQC (version 0.11.5)