FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21512337
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA20600.016.24205210
ATCACCG36150.015.5445745
CCGTGCG25450.014.6367799
TCGTTCG19150.014.250339523
CGACCTT37500.014.07353215
ACTCGAG27550.014.00433745
TCACTCG54150.013.83817745
TCAACCG81650.013.34292917
CGAGAAT24300.013.16267514
CATCGTT27800.012.84252932
CAATCGC67500.012.76024233
CGTTCGG21550.012.66544424
ACCGCCC67600.012.5349758
TCGTTGT28700.012.43968734
TTCGGTA20350.012.39792626
CGGTAAG20350.012.28627528
CGCTCTT34200.012.26600434
AACCGCC88900.011.97226319
CATACAC175400.011.9009773
TCAATCG73300.011.87233232
Produced by FastQC (version 0.11.5)