FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004723978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004723978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15116260
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA10200.015.66054410
TCGTTGT14350.015.36516534
CATCGTT15350.014.51060632
TATACTG29200.013.9458325
TCTCGTA10600.013.58429638
TATACAC196250.013.36852645
TATACCG12650.012.9831786
CGGTATG10950.012.94530943
CTCGTAT11250.012.39944539
CCGTTTA12250.012.30516413
CGAGAAT11350.012.2897914
TCGTACT9050.012.18145226
ATCGTAC9100.012.1145225
GCGAAGT12750.011.82288232
TTATACA252550.011.76037645
ATCTCGT12200.011.61833237
TCGTTCG8650.011.44427123
CATCGTA9900.011.1355724
ACGAGAC13000.011.07613422
GTATAGA29350.011.0668021
Produced by FastQC (version 0.11.5)