FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16180012
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG23200.017.4216483
GTATCGA21450.017.0900524
TATCGAA22300.017.0708395
CCCGTAT28600.016.7872311
TCGGGTC70450.016.035182
CTCGGGT93300.014.8828231
CCGTATC31950.014.4377262
CCCGTCT74150.014.2194181
CTCGTTT41150.014.06955051
TCGCGTG7850.013.1915732
CTCGGAT57700.013.05236151
CTCGCGT12500.012.8030621
CTCGGTT34000.012.4597271
CGGGTCT98550.012.4469413
CTCCGAT36700.012.312611
TCGGATT31050.012.2791132
CGCGTAT4254.3721993E-612.1828441
GTCCGAT11600.012.1732961
TGTCGCG18150.012.1702267
CCCGCAT37100.012.0529851
Produced by FastQC (version 0.11.5)