FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35558923
Sequences flagged as poor quality0
Sequence length32-101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA24100.063.72581
GTCGGAT44000.054.34341
TCGGATT51050.048.746912
GCGTAAC32100.047.6619952
AGATCGG165850.046.51695694-95
GTTATTT403350.041.1454161
GTGCGAT1058900.039.0125961
TGCGATC1081600.038.2794842
TGTATTG151500.038.1817322
GCGATCG1109650.037.328973
GTCGATT26800.036.1466521
TAGTTAG44900.035.4025279
TTATTTT473400.035.18112
GTATTGT165750.034.8422623
AAGATCG50100.034.53857494-95
CAGATCG224450.034.17773494-95
CTCGCTA309600.034.098181
CCAGGCT868500.034.0661351
GCCGTAT15750.033.6036531
TAGATCG32800.033.10920794-95
Produced by FastQC (version 0.11.5)