FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36323824
Sequences flagged as poor quality0
Sequence length32-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGT52650.038.031461
AGATCGG101000.036.2539294-95
GGTGGCG292000.031.3517972
CGGTGGC298200.031.2991711
TGGCGCG293700.031.2807714
GGCGCGT300900.030.4378365
CGCTACG55600.029.2820871
GCGCGTG328000.027.9180776
CAGATCG95350.026.4966694-95
CGACGAC543400.026.4225581
GTGGCGC350000.026.2231463
ACGACCC546300.026.0779723
GCTACGC62850.025.9628722
CGACCCA559700.025.4611784
CATTCGA593300.023.9573949
GCTCGTG86650.023.3642562
GACGACC629300.023.3071612
TGGGCGC185500.022.9559787
GCGACAT70500.022.6755777
CGCGTGC404350.022.6694767
Produced by FastQC (version 0.11.5)