FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35086275
Sequences flagged as poor quality0
Sequence length32-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT635630.1811620070811165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC386150.047.66431
GGTGGCG470900.038.0427322
TGGCGCG572300.031.2684294
AGATCGG103750.031.19573894-95
ACGTGCG66700.030.0954788
GGCGCGT632350.028.2315125
GCGCGTG656850.027.168886
GTGGCGC663750.027.1167743
GTCTAAC87400.024.5509321
TAACACG85950.023.5761644
GGCGATC58750.023.314482
CGTGCGC88800.022.9223989
GCGACAT113650.022.1196697
AACACGT94900.021.8957795
TGCGACA116100.021.49136
CGCGTGC836050.021.328647
GCGATCT68100.020.7333813
TAGATCG24850.020.64321194-95
GCGTGCC883700.020.295348
CAGATCG124600.020.09712294-95
Produced by FastQC (version 0.11.5)