FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17552028
Sequences flagged as poor quality0
Sequence length89
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10002005.698486807336452No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1152020.6563458080171705No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT601130.34248464052131183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG532500.3033837457415178No Hit
GGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGT379920.21645362006031438No Hit
GTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACC356110.2028882360488486No Hit
GTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTC316350.18023558303348194No Hit
GCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAA305460.17403117178254274No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC294590.16783815522627926No Hit
GAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGC292920.16688669822085517No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG292000.1663625422657712No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC289590.16498948155734483No Hit
GAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTA283710.16163944132267793No Hit
GTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCT275220.15680239343282726No Hit
GGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCC263080.14988581376465443No Hit
GCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTT258420.14723084990520754No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC254040.14473541177122096No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCG253580.144473333793679No Hit
GGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGC252030.14359024495630934No Hit
GTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACG233900.13326095423275305No Hit
GCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGT226620.1291132853707845No Hit
GATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGAT217340.1238261470412422No Hit
GAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCT217080.1236780160104576No Hit
AGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGG207300.11810601031402185No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC206650.11773568273706035No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT205590.11713176391924625No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC202650.11545674380191281No Hit
GTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG202630.11544534910723707No Hit
ACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGAC201350.11471608864798985No Hit
GCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTT200410.11418053799823018No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAG200200.11406089370413493No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC194260.11067666938544081No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA194180.11063109060673786No Hit
GGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTC193180.11006135587295097No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGT192000.10938906888708244No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC191640.10918396438291916No Hit
CTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAG191440.1090700174361618No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC190020.10826099411418441No Hit
GGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTT189670.108061586957359No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT189170.10777671959046556No Hit
ATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCC188700.10750894426558573No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG188540.10741778670817982No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCC187820.10700757769985325No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA185010.1054066230979121No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA184630.10519012389907309No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG182470.10395949687409341No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC178750.10184008366440618No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG177930.10137290118270093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1122500.081.9790341
AGCAGTG1211250.075.9442752
GCAGTGG1341400.068.606893
CAGTGGT1343350.068.4263844
AGTGGTA1356000.067.738885
GTGGTAT1369750.067.019326
TGGTATC1369600.066.978177
GTATCAA1371150.066.935919
GGTATCA1371350.066.9201058
CTACACG11450.064.5672451
TACACGA16150.046.0107352
ACACGAC18300.040.5942273
ACGACGC20950.035.4589925
AGTACTT1344400.035.2448820-21
AGAGTAC1367750.035.13617318-19
ACGCAGA1381950.035.12642714-15
GCAGAGT1384150.034.94462616-17
TACTTTT1352150.034.88644422-23
ATCAACG1409450.034.55614510-11
CAACGCA1413250.034.41174312-13
Produced by FastQC (version 0.11.5)