FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17239207
Sequences flagged as poor quality0
Sequence length89
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9808415.689594654788936No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1133090.6572750127079512No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT589140.3417442577260079No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG503550.29209580231851734No Hit
GGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGT381800.22147190413108908No Hit
GTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACC351270.2037622728238022No Hit
GTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTC312120.18105241151753676No Hit
GCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAA302830.17566353255112024No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC290590.1685634379818051No Hit
GAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGC288640.16743229546463476No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG287740.16691022968747926No Hit
GAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTA282040.16360381309882757No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC276110.16016397970045837No Hit
GTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCT264190.1532495085185763No Hit
GGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCC260440.15107423444709492No Hit
GCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTT250990.14559254378696188No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC249960.14499506850866167No Hit
GGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGC247110.14334186021433584No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCG246280.1428603995531813No Hit
GTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACG227770.13212324673634931No Hit
GCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGT222320.12896184841912972No Hit
GATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGAT215560.12504055435960598No Hit
GAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCT213200.12367158187728705No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT207050.1201041324000576No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC206270.1196516753931895No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC200380.11623504491824943No Hit
AGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGG199960.11599141422224352No Hit
ACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGAC198500.11514450751708011No Hit
GTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG198110.11491827901364605No Hit
GCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTT196420.11393795549876512No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAG195460.11338108533646588No Hit
GGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTC194700.11294022979131232No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC191290.11096218056897861No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT190860.1107127491421154No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA189520.10993545120723941No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC189040.10965701612608979No Hit
GGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGT188840.10954100150894412No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC188030.10907114230950415No Hit
CTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAG187940.10901893573178859No Hit
ATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCC185980.10788199248376099No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA183500.10644341123115465No Hit
GGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTT183240.10629259222886529No Hit
GGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCC183010.10615917541914777No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA181980.10556170014084755No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG181340.1051904533659814No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG178830.10373446992080321No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC175890.10202905504876181No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG175020.10152439146417815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1110050.082.092141
AGCAGTG1200950.075.864852
GCAGTGG1335600.068.269183
CAGTGGT1338700.068.027784
AGTGGTA1353650.067.2550055
GTGGTAT1362800.066.705616
TGGTATC1364150.066.6087957
GTATCAA1366050.066.554869
GGTATCA1366000.066.5481958
CTACACG11100.060.9891171
TACACGA14900.046.5244182
ACACGAC17750.039.0453573
ACGACGC19500.035.9671365
AGTACTT1340250.035.1176820-21
AGAGTAC1362800.035.06934718-19
ACGCAGA1377550.035.03420614-15
GCAGAGT1379200.034.8448616-17
TACTTTT1345700.034.7734222-23
ATCAACG1403600.034.4610710-11
CAACGCA1407350.034.33518212-13
Produced by FastQC (version 0.11.5)