FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004724409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24842065
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTCTACTCTCAGTTTACTGCTAAAT8687963.4972777021555976No Hit
CGTAGCGACGGGCGGTGTGTACAAAGGG3654131.470944545069019No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1442950.5808494583682958No Hit
GCACTTACTGGGAATTCCTCGTTCATGG1386730.5582184894854756No Hit
TTTGCGCTGTTATCCCTAGGGTAACTTG1077200.4336193468618651No Hit
ACGCCAGAGTCTCGTTCGTTATCGGAAT967080.38929130891493924No Hit
GACGCGGGCTTATGCGGAGGAGAATGTT943530.3798114206689339No Hit
TGCGTGGGGGTTTTAGTTAAATGTCCTT934680.3762489148949574No Hit
TGCGTGGGTACTTGCGCTTACTTTGTAG910320.36644296679845256No Hit
TTTGCGCTTACTTTGTAGCCTTCATCAG805910.3244134495260358No Hit
GCGCAGTTATCCCTAGGGTAACTTGTTC781740.31468398460433944No Hit
CGCTTCAGGGCCCTGTTCAACTAAGCAC747160.300764046789186No Hit
ACACGACGCTCTTCCGATCTACACGACG723590.29127610768267453No Hit
TGTGTTTTAATCTGACGCAGGCTTATGC635940.25599321151442117No Hit
CGTAGGACTTTAATCGTTGAACAAACGA605430.24371162381227165No Hit
TGCGTGGGTATAATACTAAGTTGAGATG600520.24173513755800896No Hit
GCACTCTACTGGGAATTCCTCGTTCATG544050.21900353291886163No Hit
CCTCTATATAAATGCGTAGGGGTTTTAG510280.20540965495420774No Hit
GCTTGAACGCTTTCTTAATTGGTGGCTG509500.20509567139446744No Hit
CGTAGGGGTTTTAGTTAAATGTCCTTTG466840.18792318593482465No Hit
AAGGCAGGGACTTAATCAACGCAAGCTT425820.17141087103668717No Hit
CACTCCAGTTTACTGCTAAATCCACCTT370600.14918244517917492No Hit
CGTGCGTTATCGGAATTAACCAGACAAA359240.1446095564116751No Hit
AAGGCAGAATCCAGATGCTCAAGGCCCT339460.13664725537108127No Hit
CGACCTTTGAAGTATACTTGAGGAGGGT323010.13002542260476332No Hit
AGAGCTTGAACGCTTTCTTAATTGGTGG312030.1256055001868806No Hit
GTCTCGTTCATGGGGAATAATTGCAATC303750.12227244393732968No Hit
CACAGGCTTATGCGGAGGAGAATGTTTT283790.11423768515218038No Hit
CGACCTTTCCTTATGAGCATGCCTGTGT279380.11246247041057174No Hit
GTAGCGACGGGCGGTGTGTACAAAGGGC271460.10927432965013174No Hit
CGTAGCGTTCTGGCGAGCAGTTTTGTTG260120.1047094917431381No Hit
GCACTCTAGAATAGGATTGCGCTGTTAT259280.10437135560187932No Hit
CGTAGCGGCTGCACCATCGGGATGTCCT258590.10409360091441673No Hit
CGTAGCGCGCGTGCAGCCCCGGACATCT254170.10231436074255502No Hit
ATTGGACTCTAGAATAGGATTGCGCTGT252140.1014971984011796No Hit
TGGTTGATTGTAGATATTGGGCTGTTAA251050.1010584265035938No Hit
GCACTCTAATTTTTTCAAAGTAAACGCT249400.10039423051183546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)