FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004724410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004724410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24842065
Sequences flagged as poor quality0
Sequence length89
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG954220.3841146056094773No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA582650.23454169369575353No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT523660.21079568063282983No Hit
GACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACC502970.20246706543920562No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT480790.1935386611378724No Hit
GTTAATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGGT427150.1719462532603469No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC426640.1717409563174398No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA383240.15427058901906907No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG372690.1500237601020688No Hit
GACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAA365580.14716167919212836No Hit
GCTAAAGGTTAATCACTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGC365460.1471133740290914No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA360350.1450563791697671No Hit
GCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACCTCTTGCTCA360120.14496379427394623No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT359430.14468603958648366No Hit
GTTTATTGCTAAAGGTTAATCACTGCTGTTTCCCGTGGGGGTGTGGCTAG357610.14395341128042294No Hit
GACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGGTTAGTATAGCTT344100.13851505500851075No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA342820.1379997999361164No Hit
GGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCT339930.13683645059297608No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT335450.13503305783959585No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC333700.1343286075453067No Hit
GGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCACT327740.13192945111447055No Hit
AAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCG322050.12963898130046758No Hit
GGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGG316060.12722774857887215No Hit
GTTAAACTTTCGTTTATTGCTAAAGGTTAATCACTGCTGTTTCCCGTGGG311700.12547266098852894No Hit
GCTTAGTTAAACTTTCGTTTATTGCTAAAGGTTAATCACTGCTGTTTCCC307610.12382626001501888No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA299000.1203603645671163No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG295940.11912858290967357No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT293010.1179491318455209No Hit
GCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG289360.1164798498031464No Hit
GCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATT276380.1112548413346475No Hit
AATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGGTTAG271050.10910928700975542No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT266560.10730186882612215No Hit
GGCACGAAATTGACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCG264870.10662157111335148No Hit
AAATTGACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATT263910.10623512980905572No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT261110.10510800933819309No Hit
ATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCA256230.10314359937468967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG104650.059.359211
TACACGA111350.056.106172
ACGACGC122200.050.8711055
ACACGAC126550.049.349833
CACGACG138050.045.179544
ACGCTCT148550.041.736288
GTATCAA79000.040.7108769
CGCTCTT154450.040.0882159
CGACGCT178250.034.7819066
AAGCAGT96150.033.2980041
AGTGGTA98350.032.489795
GACGCTC196000.031.7381977
GGTATCA102300.031.4790698
GTGGTAT108400.029.9371036
CAGTGGT109000.029.7714144
TGGTATC117100.027.9258257
CGGTTTT25850.022.0362031
ACGCAGA78950.021.99746314-15
CAACGCA79650.021.9606412-13
CGTTTTA12600.021.7796131
Produced by FastQC (version 0.11.5)